Library preparation and targeted sequencing for the Ion Torrent PGM was done following the manufacturer's protocol. Briefly, after DNA end repair to generate blunt ends, individual barcodes and sequencing adapters (Xpress Barcode Adapters…
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Concordance of MiSeq, pyrosequencing and Ion Torrent Personal Genome Machine (IT-PGM) findings. A representative sample in which a KRAS mutation (p.G60V) identified by pyrosequencing was also detected and called by both MiSeq and the IT-PGM…
